禽胰多肽对肉鸡小肠消化酶活力影响的研究 （19页 7038字） 摘要：禽胰多肽（avian pancreation polypeptide ，APP）是胰脏胰多肽细胞分泌的一种多肽类激素。APP具有促进畜禽采食。加快生长发育等作用。它能抑止胃肠道腺体分泌，延缓胃排空和十二指肠运动，从而使胃肠道有充分的时间来消化。本实验在自制鸡胰多肽（APP）粗品及其纯度鉴定的基础上进行了动物实验。具体实施方案如下：100只肉鸡被随机分为5组，每组20只。Ⅰ组为对照组，每天饲饮自来水。Ⅱ、Ⅲ组分别饲饮剂量为0.58 mg/d的APP粗品及剂量为0.29 mg/d的APP纯品。Ⅳ、Ⅴ组分别于每周颈部皮下注射24 µg/只的APP粗品及12µg/只APP纯品。饲养称重，宰杀后取小肠进行消化酶活力测定。结果表明：APP通过提高肉鸡小肠消化酶活力来增强机体代谢机能，从而促进肉鸡体重增加，以上结果为进一步探索禽胰多肽的生理功能提供了基础。
关键词：肉鸡；禽胰多肽；蛋白酶；淀粉酶；脂肪酶；酶活力 Effects of avian pancreatic polypeptide on edible chicken′s disgestive enzyme activity in small intestine Abstract:Avian pancreatic polypeptide（APP）is a kind polypeptide horrmore produced by pancreation polypeptide cells in pancreas. APP plays an important role in accelerating the development of birds and potentiating feeding of them.APP can suppress the secretion of gastro-intestinal tract gland. At the same time it could postpone the stomach evacuation and slow the movement of duodenum. Thus it make the gastro-intestinal tract to have enough time to digest. Based on the purification of homemade-APP sample, this study do some animal experiments to prove its functions. The research is as following: 100 chickens were randomly divided into 5 groups and every group involved 20 chickens.Control Ⅰ, Treatment Ⅱ(Purification of APP sample,0.58mg/only day), Treatment Ⅲ(fluid purification,0.29mg/only day), Treatment Ⅳ(Purification of APP sample, 24mg/only5 days), Treatment Ⅴ(fluid purification,0.29mg/only 5 days). After these feeding, all of them were weighed and smapled to get the enzyme activity from the duodenum. The results showed APP that purification of APP could improve the development of chicken and increase the efficency of the usage of forage. At last it could give a rise on the metabolism of meat-chicken from whole body and provide theoretical basis on the function of APP. key words: edible chicken; APP; protein kinase A; Amylase; lipase; Enzyme activity 目录 禽胰多肽（avain pancreatic polypeptide，APP）是在20世纪60年代末由Kimmel等在提取鸡胰岛素时偶然发现的，它由PP细胞合成，为36个氨基酸残基组成的胃肠激素，经实验研究显示：APP具有促进禽类采食，加快生长发育，调节胃肠道运动，影响体内许多激素活动，保护肝细胞完整性及细胞活性等生理作用。 。。。。。。。。。。。 [1] Kimmel J.R,Pollock H.G，and Hazelwood，R.L. Isolation and characterization of chicken insulin. Endocrinology，1968,（83）：1323～1330. [2] Marks NJ,Shaw C,et al The corrected primary structure of chicken (avian) pancreatic polypeptide.Regul Pept 1994 Aug 4;52(3):159～164. [3] Kimmel JR,et al，Gut Hormones Edinburgh,1978,234. [4] Leveille GA,et al,Proceedings of the Society for Experimental Biology and Medicine，1968,128：398 [5] 杨霖.胃肠激素对胃肠运动调节的研究进展. 阿坝科技.1998，（2）：68～72. [6] 朱晓彤，傅伟龙. 禽胰多肽对粤黄公鸡生长的影响. 华南农业大学学报. 2002，23（2）：65～69. [7] Berston G G ,Iipf W B, et al Pancreatic polypeptide infusions reduce food intake in PraderWilli syndrome. Pepties 1993 May～Jun;14(3):497～503. [8] 凌贤龙,徐纬中.胰多肽研究现状.国外医学消化系疾病分册.1992（12）3：133～136. [9] 顾增发，牛胰多肽对大鼠胰外分泌的抑制作用，生理学报，1984，（36）6：579～583. [10] 鲁立，潘国宗. 对牛胰多肽抑制胰酶分泌作用的离体研究. 生理学报. 1987，（39）1：1～9. [11] 胡望明. 胆囊运动的内分泌和神经调控. 国外医学生理病理科学与临床分册. 1996，16（4）：242～244. [12] 代建国，傅伟龙，高萍. 禽胰多肽粗品对肉鸡生长及血液生长激素、甲状腺激素水平的影响. 动物营养学报. 2000，（12）2：39～42. [13] Grobecker H. Transmitter～peptide coexistence in the central nervous system. Eur Neurol 1983;22 Suppl 1:38～46. [14] Katayama Kumoi Y, Kiyama H, et al Tohyama M. Coexistence of pancreatic polypeptide and substance P in the chicken retina. Brain Res 1985 Dec 30;361(1):25～35. [15] Lundberg JM, Hokfelt T, et al Goldstein M, Markey K. Coexistence of an avian pancreatic polypeptide (APP) immunoreactive substance and catecholamine in some peripheral and central neurons. Acta Physiol Scand 1980 Sep;110(1):107～109. [16] 杨丽，周悦等. 牛胰多肽与脂质作用时插膜状态的研究. 生物物理学报. 1997，13（2）：179～184. [17] 聂松青，庞青海等. 牛胰多肽与DPPC脂质体相互作用的傅立叶变换红外光谱研究.生物物理学报. 1989，5（2）：208～212. [18] 聂松青，林克椿，杨先春等. 牛胰多肽及去氧胆酸盐与DMPC脂质体相互作用的激光拉曼光谱研究. 生物物理学报. 1990，6（3）：244～348. [19] 宋占军,姜厚理,公衍道等. 牛胰多肽二级结构的红外光谱和圆二色谱分析. 生物化学杂志. 1995，11（6）：717～720. [20] 张晓楠,曹云新,张延风等. 用于测定小分子多肽的两种电泳方法的比较. 生物工程进展. 2001,21（3）：74～75. [21] Steinman J L. The effects of adrenergic,opioid and pancreatic polypeptidergic compounds on feeding and other behaviors in neonatal [22] Duke GE, J.R.Kimmel , Chaplin S B et al . Influence of avian pancreatic and billiary secrection in laying hens. Poultry Science. 1988，67（1）：126]～130. [23] Denbow DM,Duke GE,et al. Food intake,gastric secretion,and motility as affected by avian pancreatic polypeptide administered centrally in chickens. Peptides 1988,9(3)：449～454. |
禽胰多肽对肉鸡小肠消化酶活力影响的研究
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