| %a1%a1%a1%a1%b1%cf%d2%b5%c9%e8%bc%c6+%bf%e0%dc%f1%c2%f3%f4%ef%b6%e0%cc%c7%ce%ef%d6%ca%b5%c4%b4%bf%bb%af%d3%eb%d6%c6%b1%b8+%b9%b227%d2%b3%a1%a210976%d7%d6%3cbr%3e%a1%a1%a1%a1%0a%d5%aa+%d2%aa%a3%ba%bf%e0%dc%f1%c2%f3%cb%d7%b3%c6%bf%e0%dc%f1%a3%ac%d1%a7%c3%fb%f7%b2%f7%b0%dc%f1%c2%f3(Tartarian+Buckwheat)%a3%ac%ca%f4%cb%ab%d7%d3%d2%b6%de%a4%bf%c6(Polygonaceae)%dc%f1%c2%f3%ca%f4(Fagopyrom+esculentum)%d6%b2%ce%ef%a3%ac%be%df%d3%d0%d2%a9%ca%b3%c1%bd%d3%c3%b9%a6%c4%dc%a1%a3%c0%e0%bb%c6%cd%aa%a3%ac%b5%b0%b0%d7%d6%ca%ba%cd%b6%e0%cc%c7%c0%e0%bb%af%ba%cf%ce%ef%ca%c7%c6%e4%d6%f7%d2%aa%c9%fa%ce%ef%bb%ee%d0%d4%b3%c9%b7%d6%a3%ac%c6%e4%b8%b3%d3%e8%bf%e0%dc%f1%bd%b5%d1%aa%d1%b9%a1%a2%bd%b5%d1%aa%d6%ac%ba%cd%bd%b5%d1%aa%cc%c7%b5%c8%c9%fa%c0%ed%b9%a6%c4%dc%a1%a3%c4%bf%c7%b0%b9%fa%c4%da%b6%d4%bf%e0%dc%f1%b6%e0%cc%c7%b5%c4%d1%d0%be%bf%bd%cf%c9%d9%c7%d2%b2%bb%b9%bb%c9%ee%c8%eb%a3%ac%b1%be%c2%db%ce%c4%d2%d4%bf%e0%dc%f1%c2%f3%f4%ef%ce%aa%d4%ad%c1%cf%bd%f8%d0%d0%b6%e0%cc%c7%b5%c4%b7%d6%c0%eb%b4%bf%bb%af%a3%ac%b2%a2%b6%d4%b4%d6%b6%e0%cc%c7%b5%c4%b4%bf%bb%af%d7%e9%b7%d6%bd%f8%d0%d0%b7%d6%ce%f6%d2%d4%d1%d0%be%bf%c6%e4%c9%fa%c0%ed%b9%a6%c4%dc%a1%a3%3cbr%3e%a1%a1%a1%a1%0a++++%d2%d4%bf%e0%dc%f1%c2%f3%f4%ef%ce%aa%d4%ad%c1%cf%bd%f8%d0%d0%bc%ee%c8%dc%d0%d4%b6%e0%cc%c7(ATP)%b5%c4%d6%c6%b1%b8%a3%acATP%be%adDEAE-Sepharose+CL-6B%c0%eb%d7%d3%bd%bb%bb%bb%d6%f9%b2%e3%ce%f6%b7%d6%bc%b6%ba%f3%b5%c3%b5%bd2%b8%f6%b6%e0%cc%c7%d7%e9%b7%d6%a3%baATP1%bc%b0ATP2%a1%a3ATP1%b5%c4%b6%e0%cc%c7%ba%ac%c1%bf%ce%aa85.3%25%a3%ac%bd%cf%b4%d6%cc%c7%b4%bf%b6%c864.53%25+%d3%d0%cb%f9%cc%e1%b8%df%a1%a3ATP1%be%adSepharose+CL-6B%c4%fd%bd%ba%d6%f9%b2%e3%ce%f6%ba%f3%bd%ab%b7%d6%d7%d3%c1%bf%b2%bb%cd%ac%b5%c4%d7%e9%b7%d6%d3%d6%bd%f8%d2%bb%b2%bd%b7%d6%bc%b6%a1%a3%3cbr%3e%a1%a1%a1%a1%0a%b2%c9%d3%c3%c6%f8%cf%e0%c9%ab%c6%d7%a3%a8GC%a3%a9%a3%ac%b8%df%d0%a7%d2%ba%cf%e0%c9%ab%c6%d7%a3%a8HPLC%a3%a9%a3%ac%ba%ec%cd%e2%b9%e2%c6%d7%a3%a8IR%a3%a9%a3%ac%d7%cf%cd%e2%b9%e2%c6%d7%a3%a8UV%a3%a9%b5%c8%cf%d6%b4%fa%d2%c7%c6%f7%b7%d6%ce%f6%ca%d6%b6%ce%a3%ac%b6%d4%bf%e0%dc%f1%c2%f3%f4%ef%b6%e0%cc%c7%b5%c4%b7%d6%bc%b6%b4%bf%bb%af%d7%e9%b7%d6%bd%f8%d0%d0%c0%ed%bb%af%d0%d4%d6%ca%b5%c4%b3%f5%b2%bd%b7%d6%ce%f6%a1%a3%bd%e1%b9%fb%b1%ed%c3%f7%a3%baATP1%b5%c4%c6%bd%be%f9%b7%d6%d7%d3%c1%bf%ce%aa8.5%26%23215%3b104+Da%a3%ac%d6%f7%d2%aa%d3%c9%c6%cf%cc%d1%cc%c7%a3%a8Glc%a3%a9%bc%b0%bc%ab%c9%d9%c1%bf%b5%c4%a3%a8%c4%be%cc%c7%a3%a9Xyl%d7%e9%b3%c9%a3%ac%b6%fe%d5%df%b5%c4%c4%a6%b6%fb%b1%c8%ce%aa9.47%3a1%a1%a3%3cbr%3e%a1%a1%a1%a1%0a%b9%d8%bc%fc%b4%ca%3a++%bf%e0%dc%f1%c2%f3++%b6%e0%cc%c7++%b4%bf%bb%af++%d7%e9%b7%d6%b7%d6%ce%f6%3cbr%3e%a1%a1%a1%a1%0aPurification+and+Preparation+of+%3cbr%3e%a1%a1%a1%a1%0aPolysaccharides+from+Tartary+Buckwheat%3cbr%3e%a1%a1%a1%a1%0aBai++Jie%3cbr%3e%a1%a1%a1%a1%0a++++(Department+of+Life-science%2c+Yuncheng+University%2c+Yuncheng%2c+shanxi%2c+044000)%3cbr%3e%a1%a1%a1%a1%0aAbstract%3a+Tartary+buckwheat%a1%afs+scientific+name+is+Tartarian+Buckwheat%2c+which+belongs+to+Polygonaceae+crop.+It+is+a+plant+having+medical+and+food+function.+Flavonoid%2c+protein+and+carbohydrate+compound+are+the+most+important+bioactive+components+which+can+lower+blood+pressure%2c+hypolipidemic+and+physiological%2c+so+it+is+called+%26quot%3bthree-drop+food%26quot%3b.+Until+now%2c+studies+about+polysaccharide+from+tartary+buckwheat+are+few+in+china.+This+paper+was+mainly+about+the+method+of+purification+of+the+polysaccharide+from+tartary+buckwheat%2c+and+it+also+analyzed+the+physiological+functions+of+the+purified+fraction+of+the+crude+polysaccharide.++%3cbr%3e%a1%a1%a1%a1%0aUsing+tartary+buckwheat+bran+prepared+for+the+alkali-soluble+polysaccharide+(ATP)%2c+ATP+was+fractionated+into+2+fractions+by+anion-exchange+chromatography+on+DEAE-Sepharose+CL-6B%3a+the+content+of+ATP1+is+85.3%25.+Compared+to+the+crude+polysaccharide+which+is+64.53%25%2c+the+content+of+the+purified+component+was+arisen%2c+ATP1+was+purified+by+Sepharose+CL-6B+again+and+separated+according+to+the+Mw.%3cbr%3e%a1%a1%a1%a1%0a+++A+series+of+modern+analysis+methods%2c+such+as+GC%2c+HPLC%2c+IR%2c+UV+were+used+to+analyze+the+main+physicochemical+properties+of+purified+fraction+of+tartary+buckwheat%a1%afs+crude+polysaccharide.+The+results+showed+that%3a+tartary+buckwheat+bran+contained+plenty+of+Glc%2c+and+little+Xyl.+The+molar+ratio+is+9.47%3a1.%3cbr%3e%a1%a1%a1%a1%0aKeywords%3a++Tartary+buckwheat++++Polysaccharide++++Purification++++Component+analysis%3cbr%3e%a1%a1%a1%a1%0a%c4%bf%c2%bc%3cbr%3e%a1%a1%a1%a1%0a%b5%da1%d5%c2++%d0%f7%c2%db%095%3cbr%3e%a1%a1%a1%a1%0a1.1%bf%e0%dc%f1%bc%f2%bd%e9%095%3cbr%3e%a1%a1%a1%a1%0a1.1.1%bf%e0%dc%f1%b5%c4%b7%d6%b2%bc%095%3cbr%3e%a1%a1%a1%a1%0a1.1.2%bf%e0%dc%f1%b5%c4%c9%fa%ce%ef%d1%a7%cc%d8%d0%d4%095%3cbr%3e%a1%a1%a1%a1%0a1.1.3%bf%e0%dc%f1%b5%c4%d3%aa%d1%f8%cc%d8%d0%d4%096%3cbr%3e%a1%a1%a1%a1%0a1.1.4%bf%e0%dc%f1%b5%c4%ca%d0%b3%a1%c7%b0%be%b0%096%3cbr%3e%a1%a1%a1%a1%0a1.2%b6%e0%cc%c7%b5%c4%d1%d0%be%bf%b8%c5%bf%f6%097%3cbr%3e%a1%a1%a1%a1%0a1.2.1%b6%e0%cc%c7%b5%c4%b7%d6%c0%e0%097%3cbr%3e%a1%a1%a1%a1%0a1.2.2%b6%e0%cc%c7%b5%c4%cc%e1%c8%a1%097%3cbr%3e%a1%a1%a1%a1%0a1.2.3%b6%e0%cc%c7%b5%c4%b7%d6%bc%b6%b4%bf%bb%af%098%3cbr%3e%a1%a1%a1%a1%0a1.2.4%b6%e0%cc%c7%b5%c4%b4%bf%b6%c8%bc%f8%b6%a8%098%3cbr%3e%a1%a1%a1%a1%0a1.3%bf%e0%dc%f1%b6%e0%cc%c7%b5%c4%d1%d0%be%bf%bd%f8%d5%b9%099%3cbr%3e%a1%a1%a1%a1%0a1.4%c1%a2%cc%e2%b1%b3%be%b0%ba%cd%d2%e2%d2%e5%099%3cbr%3e%a1%a1%a1%a1%0a1.5%b1%be%bf%ce%cc%e2%b5%c4%d6%f7%d2%aa%d1%d0%be%bf%c4%da%c8%dd%0910%3cbr%3e%a1%a1%a1%a1%0a%b5%da2%d5%c2++%b2%c4%c1%cf%d3%eb%b7%bd%b7%a8%0911%3cbr%3e%a1%a1%a1%a1%0a2.1%b2%c4%c1%cf%d3%eb%c9%e8%b1%b8%0911%3cbr%3e%a1%a1%a1%a1%0a2.1.1%d4%ad%c1%cf%d3%eb%ca%d4%bc%c1%0911%3cbr%3e%a1%a1%a1%a1%0a2.1.2%d2%c7%c6%f7%d3%eb%c9%e8%b1%b8%0911%3cbr%3e%a1%a1%a1%a1%0a2.2%ca%b5%d1%e9%b7%bd%b7%a8%0911%3cbr%3e%a1%a1%a1%a1%0a2.2.1%bc%ee%cc%e1%b4%d6%b6%e0%cc%c7ATP%b5%c4%cc%e1%c8%a1%b9%a4%d2%d5%0911%3cbr%3e%a1%a1%a1%a1%0a2.2.2+%bf%e0%dc%f1%b6%e0%cc%c7%b5%c4%b3%ac%c2%cb%c5%a8%cb%f5%0912%3cbr%3e%a1%a1%a1%a1%0a2.2.3%bf%e0%dc%f1%b6%e0%cc%c7DEAE-Sepharose+CL-6B%d6%f9%b2%e3%ce%f6%a3%a8D+2.6%26%23215%3b30+cm%a3%a9%b7%d6%bc%b6%0912%3cbr%3e%a1%a1%a1%a1%0a2.2.4%bf%e0%dc%f1%b6%e0%cc%c7Sepharose+CL-6B%d6%f9%b2%e3%ce%f6%a3%a8D+1.0cm%26%23215%3b120+cm%a3%a9%b7%d6%bc%b6%0912%3cbr%3e%a1%a1%a1%a1%0a2.2.5%bf%e0%dc%f1%b6%e0%cc%c7%b5%c4%ba%ac%c1%bf%b2%e2%b6%a8%a3%a8%b1%bd%b7%d3%a3%ad%c1%f2%cb%e1%b7%a8%a3%ac%d2%d4%c6%cf%cc%d1%cc%c7%ce%aa%b1%ea%d7%bc%a3%a9%0912%3cbr%3e%a1%a1%a1%a1%0a2.2.6%bf%e0%dc%f1%b6%e0%cc%c7%b5%c4%b5%a5%cc%c7%d7%e9%b3%c9%b7%d6%ce%f6%0913%3cbr%3e%a1%a1%a1%a1%0a2.2.7%bf%e0%dc%f1%b6%e0%cc%c7%b5%c4%cf%e0%b6%d4%b7%d6%d7%d3%c1%bf%b7%d6%b2%bc%ba%cd%b4%bf%b6%c8%bc%f8%b6%a8%0913%3cbr%3e%a1%a1%a1%a1%0a2.2.8%bf%e0%dc%f1%b6%e0%cc%c7%b5%c4%ba%ec%cd%e2%b9%e2%c6%d7%a3%a8FT-IR%a3%a9%b7%d6%ce%f6%0914%3cbr%3e%a1%a1%a1%a1%0a2.2.9%bf%e0%dc%f1%b6%e0%cc%c7%b5%c4%d7%cf%cd%e2%b9%e2%c6%d7%a3%a8UV%a3%a9%b7%d6%ce%f6%0914%3cbr%3e%a1%a1%a1%a1%0a%b5%da3%d5%c2++%bd%e1%b9%fb%d3%eb%cc%d6%c2%db%0915%3cbr%3e%a1%a1%a1%a1%0a3.1%b3%ac%c2%cb%c5%a8%cb%f5%0915%3cbr%3e%a1%a1%a1%a1%0a3.2%bf%e0%dc%f1%b6%e0%cc%c7%b5%c4%c0%eb%d7%d3%bd%ba%d6%f9%b2%e3%ce%f6%0915%3cbr%3e%a1%a1%a1%a1%0a3.3%bf%e0%dc%f1%b6%e0%cc%c7%b5%c4%c4%fd%bd%ba%d6%f9%b2%e3%ce%f6%0916%3cbr%3e%a1%a1%a1%a1%0a3.4%b6%e0%cc%c7%ba%ac%c1%bf%b5%c4%b2%e2%b6%a8%0916%3cbr%3e%a1%a1%a1%a1%0a3.5%b5%a5%cc%c7%d7%e9%b3%c9%b7%d6%ce%f6%0918%3cbr%3e%a1%a1%a1%a1%0a3.6%ba%ec%cd%e2%b9%e2%c6%d7%b7%d6%ce%f6%a3%a8IR%a3%a9%0920%3cbr%3e%a1%a1%a1%a1%0a3.7%d7%cf%cd%e2%b9%e2%c6%d7%b7%d6%ce%f6%a3%a8UV%a3%a9%0920%3cbr%3e%a1%a1%a1%a1%0a3.8+HPLC%c4%fd%bd%ba%c9%ab%c6%d7%b7%d6%ce%f6%0921%3cbr%3e%a1%a1%a1%a1%0a%b5%da4%d5%c2++%bd%e1%c2%db%0924%3cbr%3e%a1%a1%a1%a1%0a%d6%c2+%d0%bb%0925%3cbr%3e%a1%a1%a1%a1%0a%b2%ce%bf%bc%ce%c4%cf%d7%0926 |
查看评论
已有0位网友发表了看法